Full Title: Hepatoprotective Activity of Easter Lily (Lilium longiflorum Thunb.) Bulb Extracts
Journal: Journal of Agricultural and Food Chemistry
Year of Publication: 2015
Publication Author(s): Wenping Tang, John P Munafo, Kimberly Palatini, Debora Esposito, Mou-Tuan Huang, Slavko Komarnytsky, Chi-Tang Ho, and Thomas J. Gianfagna
The hepatoprotective activity of two different extracts, a hydroethanolic crude bulb extract (CB) and a steroidal glycoside-rich 1-butanol extract (BuOH), prepared from the bulbs of Easter lily (Lilium longiflorum Thunb.), were evaluated in a 24 week study in the female KK.Cg-Ay/J Type 2 diabetic mouse model. Animals were divided in six groups (n=16): control mice received Easter lily bulb extract-free drinking water together with a low or high fat diet (diabetic control); drinking water for the remaining groups was supplemented with CB extract (1%), BuOH extract (0.1% or 0.2%) and a reference drug Metformin (0.001%), together with a high fat diet. Both CB and BuOH extract treatment groups exhibited significantly improved liver function based on comparisons of triglycerides (diabetic 219 ± 34, CB 131± 27, BuOH(0.2%) 114 ± 35 mg/dL), CB total cholesterol (TC) (diabetic 196 ± 12, CB 159 ± 5 mg/dL), average liver mass (diabetic 2.96 ± 0.13, CB 2.58 ± 0.08, BuOH(0.1%) 2.48 ± 0.13 g), alanine transferase (diabetic 74 ± 5, CB 25 ± 1, BuOH(0.1%) 45 ±1 units/L), and histological examinations. Improved glucose metabolism was observed only in CB, which was confirmed by oral glucose tolerance tests (OGTT) in diet-induced obese C57BL/6J mice exposed to CB extract. These data suggest that steroidal glycosides 1 – 5 may play a role in the hepatoprotective activity of the two BuOH extracts, while the results of the TC measurements and OGTT study indicate that other constituents present in the CB extract are responsible for its hypocholesterolemic and hypoglycemic activity.