Cytotoxic effects of ellagitannins isolated from walnuts in human cancer cells

Full Title: Cytotoxic effects of ellagitannins isolated from walnuts in human cancer cells

Journal: Nutrition and Cancer

Year of Publication: 2014

PHHI Author(s): Mary Grace Mary Ann Lila Slavko Komarnytsky
Publication Author(s): Le V, Esposito D, Grace M, Ha D, Pham A, Bortolazzo A, Bevens Z., Kim J, Okuda R, Komarnytsky S, Lila MA, White B

Abstract:

Walnuts contain many bioactive components that may slow cancer growth. A previous report showed that a diet supplemented with walnuts decreased the tumor size formed by MDA-MB-231 human cancer cells injected into nude mice. However, the mechanism of action was never determined. We characterized the effects of a methanol extract prepared from walnuts on human MDA-MB-231, MCF7, and HeLa cells. The extract was cytotoxic to all cancer cells. We identified compounds from the methanol extract that induced this cytotoxicity. The predominant compounds were Tellimagrandin I and Tellimagrandin II, members of the ellagitannin family. We also show a walnut extract decreases the intracellular pH, depolarizes the mitochondrial membrane with release of cytochrome c and phosphatidylserine flipping. The antimitogenic effects of walnut extract were associated with a twofold reduction of mitochondria respiration. These results suggest impairment of mitochondrial function and apoptosis as relevant mechanism of anticancer effects of the walnut extract.

Link to Article: http://www.tandfonline.com/doi/full/10.1080/01635581.2014.956246#abstract