Brassinosteroid enhances C57BL/6J mice treadmill endurance

Full Title: Brassinosteroid enhances C57BL/6J mice treadmill endurance

Journal: FASEB J

Year of Publication: 2012

PHHI Author(s): Slavko Komarnytsky
Publication Author(s): Esposito D, Tuazon M, Henderson GC, Komarnytsky S, Raskin I


The 28-homobrassinolide (HB) has been show to have multiple anabolic effects on protein synthesis and lean body mass in healthy rats. Using 7 wk old male C57BL/6J mice, we examined the effects of 28 d of HB treatment on grip strength, exercise performance, and HB-gene interactions in mouse hindlimb muscles. Animals received 60 mg/kg of HB in corn oil (vehicle) via oral gavage daily and underwent bi-weekly treadmill exercise on d 1 (baseline), 14 and 28 of the experiment. Significant increases in treadmill performance and enhanced grip strength were observed with HB. Components of PI3K/Akt, AMPK, and myogenesis signaling pathways were significantly upregulated by HB treatment in skeletal muscle tissues. These findings indicate that brassinosteroid alone induces an integrated response that includes structural and metabolic changes in skeletal muscles resulting in greater endurance capacity. This study was supported in part by the 5P50AT002776-05 grant from NCCAM.

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