Biochemical analysis and in vivo hypoglycemic activity of grape polyphenol – soybean flour complex

Full Title: Biochemical analysis and in vivo hypoglycemic activity of grape polyphenol - soybean flour complex

Journal: Journal of Agricultural and Food Chemistry

Year of Publication: 2012

PHHI Author(s): Mary Ann Lila
Publication Author(s): Roopchand, Diana E., Peter Kuhn, Alex Poulev, Andrew Oren, Mary Ann Lila, Ilya Raskin

Abstract:

Abstract Image

Defatted soybean flour (DSF) can efficiently sorb, concentrate, and stabilize polyphenols, but not sugars, from Concord grape juice, to yield grape polyphenol-enriched DSF. Sorption of grape polyphenols to DSF particles was dependent on the ratio of DSF and grape juice concentrate used, but not time of mixing or pH. Depending on ratios of starting materials, 1 g of grape polyphenol-enriched DSF contained 1.6–10.4 mg of anthocyanins, 7.5–93.1 mg of proanthocyanidins, and 20.5–144.5 mg of total polyphenols. LC-MS analysis of grape juice samples before and after addition and removal of DSF and eluate from grape polyphenol-enriched DSF confirmed that a broad range of grape compounds were sorbed to the DSF matrix. Finally, grape polyphenol-enriched DSF was able to significantly lower blood glucose levels in hyperglycemic C57BL/6J mice. The data indicate that grape polyphenol-enriched DSF can provide a high-protein, low-sugar ingredient for delivery of concentrated grape polyphenolics.

Link to Article: http://pubs.acs.org.prox.lib.ncsu.edu/doi/ipdf/10.1021/jf300232h