A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution

Full Title: A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution

Journal: Nature Genetics

Year of Publication: 2016

PHHI Author(s): Massimo Iorizzo
Publication Author(s): Massimo Iorizzo, Shelby Ellison, Douglas Senalik, Peng Zeng, Pimchanok Satapoomin, Jiaying Huang, Megan Bowman, Marina Iovene, Walter Sanseverino, Pablo Cavagnaro, Mehtap Yildiz, Alicja Macko-Podg├│rni, Emilia Moranska, Ewa Grzebelus, Dariusz Grzebelus, Hamid Ashrafi, Zhijun Zheng, Shifeng Cheng, David Spooner, Allen Van Deynze & Philipp Simon

Abstract:

We report a high-quality chromosome-scale assembly and analysis of the carrot (Daucus carota) genome, the first sequenced genome to include a comparative evolutionary analysis among members of the euasterid II clade. We characterized two new polyploidization events, both occurring after the divergence of carrot from members of the Asterales order, clarifying the evolutionary scenario before and after radiation of the two main asterid clades. Large- and small-scale lineage-specific duplications have contributed to the expansion of gene families, including those with roles in flowering time, defense response, flavor, and pigment accumulation. We identified a candidate gene, DCAR_032551, that conditions carotenoid accumulation (Y) in carrot taproot and is coexpressed with several isoprenoid biosynthetic genes. The primary mechanism regulating carotenoid accumulation in carrot taproot is not at the biosynthetic level. We hypothesize that DCAR_032551 regulates upstream photosystem development and functional processes, including photomorphogenesis and root de-etiolation.

Link to Article: http://www.nature.com/ng/journal/vaop/ncurrent/full/ng.3565.html