α-Amylase and α-Glucosidase Inhibitory Activities of Phenolic Extracts from Eucalyptus grands x E. urophylla Bark

Full Title: α-Amylase and α-Glucosidase Inhibitory Activities of Phenolic Extracts from Eucalyptus grands x E. urophylla Bark

Journal: Journal of Chemistry

Year of Publication: 2017

PHHI Author(s): Mary Grace Mary Ann Lila
Publication Author(s): Jiang, P., Xiong, J., Wang, F., Grace, M.H., Lila, M.A., Xu, R.

Abstract:

This study evaluated the inhibitory effects of different extracts and fractions from
Eucalyptus. grandis × urophylla bark (EB) against α-glucosidase and α-amylase enzyme activities. The ethyl acetate extract (EB-E) showed the highest activity among others. Seven fractions were derived from EB-E; among them EB-E-7 showed the highest significant inhibition of both enzymes, with IC 50 of 1.40 ± 0.18 and 1.72 ± 0.12 μg/mL, respectively. EB-E and its active fraction EB-E-7 showed highest contents of total phenolics: 178.79 ± 4.68 and 920.4 ± 5.46 mg GAEag−1, respectively. HPLC-MS analysis of EB-E-7 revealed the presence of ellagic acid, quercetin-glucuronide, quercetin-3-α-rhamnopyranoside, and ellagic acid rhamnoside as major compounds, together with smaller concentrations of myricetin-rhamnoside, isorhamnetin-hexoside, myricetin-3-
α-arabinofuranoside, and isorhamnetin. Therefore, the phenolic compounds from
Eucalyptus grandis×E. urograndis bark potently inhibited α-amylase and α-glucosidase activity, having potential in prevention of hyperglycemia.

Link to Article: http://downloads.hindawi.com/journals/jchem/2017/8516964.pdf