Gad Yousef

Gad Yousef in the research field
Senior Researcher
Department of Food, Bioprocessing and Nutrition Sciences

Ph.D., University of Illinois at Urbana-Champaign
704-250-5431
ggyousef@ncsu.edu

Dr. Gad Yousef works in the laboratories of Dr. Mary Ann Lila and Dr. Allan Brown. His research interests include biosynthesis of radiolabeled flavonoids using cell culture systems and photochemical characterization of secondary metabolites in plants. He also contributes to the breeding efforts of fruits and vegetables, such as blueberry, broccoli, tomato and cabbage, for enhanced human health benefits. Dr. Yousef advises graduate and post-doctoral students on research related to his areas of expertise.

Publications

Cheng, Diana M., Gad G. Yousef, and Mary Ann Lila. 2010. Variation in phytoecdysteroid accumulation in seeds and shoots of Spinacia oleracea L. accessions. Hort Science 45: 1-5.

Cuevas-Rodriguez, Edith, Gad G. Yousef, Pedro A. Garcia-Saucedo, Jose Lopez-Medina, Octavio Paredes-Lopez, and Mary Ann Lila. 2010. Characterization of anthocyanins and proanthocyanidins in wild and domesticated Mexican blackberries (Rubus spp.). Journal of Agricultural and Food Chemistry 58:7458-7464.

Maria Elisa Schreckinger, Jinzhi Wang, Gad Yousef, Mary Ann Lila and Elvira Gonzalez de Mejia. 2010. Antioxidant capacity and in vitro inhibition of adipogenesis and inflammation by phenolic extracts of Vaccinium floribundum and Aristotelia chilensis. Journal of Agricultural and Food Chemistry 58:8966-8976.

Mun, Jonathan G., Michael D. Grannan, Pamela J. Lachcik, Randy B. Rogers, Gad G. Yousef, Mary H. Grace, Elsa M. Janle, Qing Li Wu, James E. Simon, Connie M. Weaver, and Mary Ann Lila. 2010. Tracking deposition of a 14C-radiolabeled hairy root-derived isoflavone-rich fraction into bone. Experimental Biology and Medicine 235: 1224-1235.

Cuevas-Rodriguez, Edith, Vermont Dia, Gad G. Yousef, Pedro A. Garcia-Saucedo, Jose Lopez-Medina, Octavio Paredes-Lopez, Elvira de Mejia and Mary Ann Lila. 2010. Inhibition of pro-inflammatory responses and antioxidant capacity of Mexican blackberry (Rubus spp) extracts. Journal of Agricultural and Food Chemistry 58: 9542-9548.

Newell, Amanda M., Gad Yousef, Mary Ann Lila, Mardco Vinicio Ramirez-Mares, and Elvira Gonzalez de Mejia. 2010. Comparative study of in vitro activities of tea extracts from six species of Ardisia and their effect on growth inhibition of HepG2 cells. Journal of Ethnopharmacology 130: 536-544.

Jahnle, Elsa, Mary Ann Lila, Michael Grannan, Lauren Wood, Aine Higgins, Gad G. Yousef, Randy B. Rogers, Helen Kim, George Jackson, and Connie M. Weaver. 2010. Method for evaluating the potential of 14C labeled plant polyphenols to cross the blood-brain barrier using accelerator mass spectrometry. Nuclear Methods and Instruments in Physics Research (NIMB) 268:1313-1316.

Janle, Elsa M., Mary Ann Lila, Michael Grannan, Lauren Wood, Aine Higgins, Gad. G. Yousef, Randy B. Rogers, Helen Kim, George S. Jackson, Lap Ho, and Connie Weaver. 2010. Pharmacokinetics and tissue distribution 14C-labeled grape polyphenols in the periphery and the central nervous system following oral administration. Journal of Medicinal Food 13:1-8.

Grace, Mary H., David M. Ribnicky, Peter Kuhn, Alexander Poulev, Sithes Logendra Gad G. Yousef, Ilya Raskin, Mary Ann Lila.  2009. Hypoglycemic activity of a novel anthocyanin-rich formulation from lowbush blueberry, Vaccinium angustifolium Aiton.  Phytomedicine 16:406-415.

Englemann, Nancy J., Randy Rogers, Gad G. Yousef, and Mary Ann Lila.  2009.  In vitro production of radiolabeled red clover (Trifolium pratense) isoflavones.  Plant Cell Tissue and Organ Culture 98:147-156.

Mun, Jon, , M.C. Grannan, P.J. Lachcik, N.A. Reppert, G. G. Yousef, R.B. Rogers, E.M. Janle, C.M. Weaver, M.A. Lila.  2009.  Metabolic tracking of radiolabeled kudzu and red clover extracts in vivo.  British Journal of Nutrition 102: 1523–1530.

Grace, Mary, Gad G. Yousef, A. Kurmukov, Ilya Raskin, and Mary Ann Lila.  2009.  Phytochemical characterization and antiyhypoxic efficacy of an active extract of Rhodiola heterodonta.  Natural Product Communications 4:1053-1058.

Reppert, Adam, G. Yousef, R. Rogers, and M. A. Lila. 2008. Isolation of radiolabeled isoflavones from kudzu (Pueraria lobata) root cultures. Journal of Agricultural and Food Chemistry 56: 7860-7865

Aparicio-Fernández, X., R. Reynoso-Camacho, E. Castaño-Tostado, T. García-Gasca, E.G. de Mejía, H. Guzmán-Maldonado, G. Elizondo-Azuela, G.G. Yousef, M.A. Lila, G. Loarca-Pina. 2008. Antiradical capacity and induction of apoptosis in HeLa cells by a Phaseolus vulgaris extract. Plant Foods for Human Nutrition 63:35-40

Cheng, D., G.G. Yousef, M.H. Grace, R.B. Rogers, J. Gorelick-Feldman, I. Raskin, and M.A. Lila. 2008. In vitro production of metabolism-enhancing phytoecdysteroids from Ajuga turkestanica. Plant Cell Tissue and Organ Culture 93:73-83.

Alwerdt, J.L., D.S. Seigler, E.G. De Mejia, G.G. Yousef, and M.A. Lila. 2008. Influence of Alternative liquid chromatography techniques on the chemical complexity and bioactivity of isolated proanthocyanidin mixtures. Journal of Agricultural and Food Chemistry. 56:1896-1906.

Shibli, R.A., M. Kushad, G.G.Yousef, and M.A. Lila. 2007. Physiological and biochemical responses of tomato microshoots to induced salinity stress with associated ethylene accumulation. Plant Growth Regulation 51:159-169.

Yousef, G.G., M.H. Grace, D.M. Cheng, I.V. Belolipov, I. Raskin, M.A. Lila. 2006. Comparative phytochemical characterization of three Rhodiola species. Phytochemistry 67:2380-2391.

Sueiro, L., G.G. Yousef, D.S. Seigler, E.G. de Mejia, M.H. Grace, and M.A. Lila. 2006. Chemopreventive potential of flavonoid extracts from plantation-bred and wild Aronia melanocarpa (Black Chokeberry) fruits. Journal of Food Science 71:C480-C4888.

Aparicio-Fernandez, X., T. Garcia-Gasca, G.G. Yousef, M.A. Lila, E.G. de Mejia, and G. Loarca-Pina. 2006. Chemopreventive activity of polyphenolics from black Jamapa bean (Phaseolus vulgaris L.) on HeLa and HaCaT cells. Journal of Agricultural and Food Chemistry 54:2116-2122.

Aparicio-Fernandez, X., G.G. Yousef, G. Loarca-Pina, E.G. de Mejia, and M.A. Lila. 2005. Characterization of proanthocyanidins in the seed coat of black Jamapa bean (Phaseolus vulgaris L.). Journal of Agricultural and Food Chemistry 53: 4615-4622.

Reyes-Carmona, J., G.G. Yousef, R. Martinez-Peniche, and M.A. Lila. 2005. Antioxidant capacity of fruit extracts of blackberry (Rubus sp.) produced in different climatic regions. Journal of Food Science 70:S497-503.

Lila, M.A., G.G. Yousef, Y. Jiang, and C. M. Weaver. 2005. Sorting out bioactivity in flavonoid mixtures. Journal of Nutrition 135: 1231-1235.

Kraft, T.F., B. Schmidt, G.G. Yousef, C.T. Knight, M. Cuendet, H. kang, J.M. Pezzuto, D.S. Seigler, and M.A. Lila. 2005. Chemopreventive potential of wild blueberry fruits in multiple stages of carcinogenesis. Journal of Food Science 70:S159-S166.

Yousef, G.G., D.S. Seigler, M.A. Grusak, R.R. Rogers, C.T. Knight, T.F. Kraft, J.W. Erdman Jr., and M.A. Lila. 2004.  Biosynthesis and characterization of 14C-enriched flavonoid fractions from plant cell suspension cultures. Journal of Agricultural and Food Chemistry 52: 1138-1145.

Grusak, M.A., R.R. Rogers, G.G. Yousef, J.W. Erdman Jr., and M.A. Lila. 2004. An enclosed-chamber labeling system for the safe 14C-enrichement of phytochemicals in plant cell suspension cultures.  In vitro Cellular and Developmental Biology-Plant 40: 80-85.

Juvik, J.A., G.G. Yousef, Y. Tadmor, T. Han, F. Azanza, W.F. Tracy, A. Bar-Zur and T.R. Rocheford. 2003. QTL influencing kernel chemical composition and seedling stand establishment in maize with the shrunken2 and sugary enhancer1 endosperm mutations. Journal of the American Society for Horticultural Sciences 128: 864-875.

Brown, A.F., G.G. Yousef, E.H. Jeffery, B.P. Klein, M.M. Kushad, M.A. Wallig, and J.A. Juvik. 2002. Glucosinolate profiles in broccoli (Brassica oleracea L.): Stability over environments and implications for cancer chemoprotection. Journal of the American Society for Horticultural Sciences 127: 807-813.

Yousef, G.G., and J.A. Juvik. 2002. Enhancement of seedling emergence in sweet corn by marker-assisted backcrossing of beneficial QTL. Crop Science 42:96-104.

Tadmor, Y., W.F. Tracy, G.G. Yousef, J.A. Juvik, and V. Raboy. 2001. low phytic acid-1 does not affect sugar metabolism in sugary1 kernels. Maydica 46: 11-19.

Yousef, G.G., and J.A. Juvik. 2001. Comparison of phenotypic and marker-assisted selection for quantitative traits in sweet corn. Crop Science 41: 645-655.

Yousef, G.G., and J.A. Juvik. 2001. Evaluation of breeding utility of a chromosomal segment from Lycopersicon chmielewskii that enhances cultivated tomato soluble solids. Theoretical & Applied Genetics 103: 1022-1027.

Site Links: Home | About | Research | Extension | Employment | Contact | Directions